Selected Current & Past Projects

Antibiotic Stewardship Initiatives 

Current Projects

In conjunction with colleagues at Keegan Mason & Associates, Crossover Biomedical is working on the following projects:

Biofire Investigator-Initiated Study Program                  Keegan, PI

Assessing the beneficial effects of viral diagnostic technology on antibiotic preservation and prevention of resistant bacteria infections in outpatient settings

  • Implementation of molecular diagnostic technology to augment existing antibiotic stewardship efforts 

Research Initiatives

Current Projects: 

In conjunction with Dr. Linda C. DeVeaux at New Mexico Institute of Mining and Technology and her students, Crossover Biomedical is working on the following projects.

NM-INBRE SBC Pilot Proposal                                     DeVeaux, PI 

Evolution of emerging pathogens in South Dakota waterways. 

  • Whole genome sequencing of selected multiply-antibiotic resistant isolates from fecally contaminated waters in South Dakota

  • Analysis and identification of genetic determinants for comparison to clinical isolates from SD DoH

NM-INBRE Focus Research Project, Pending              DeVeaux, PI

Effects of wastewater treatment processes on the differential survival of potentially pathogenic, antibiotic resistant bacteria.  

  • Analysis of the resistome within wastewater at various stages of the treatment processes 

  • Comparison of activated sludge process to fixed film plant for selection of resistant microbes

 

Invited Lectures

South Dakota Water and Wastewater Association: 2018 Annual Wastewater Seminar.  Path-STREAM: A Tool for Assessing Pathogenic Potential of Bacterial Communities within Water Environments.  Murray, K.E., DeVeaux, L.C.  Pierre, SD.  April 12, 2018.  

                                            

30th Annual Environmental and Ground Water Quality Conference, SD Department of Environment and Natural Resources.  Pathogens in Various Waterways Across the State of South Dakota.  Murray, K.E., DeVeaux, L.C.  Pierre, SD.  March 28, 2018

Rapid Creek Workshop at Outdoor Campus West.  Pathogenic Bacteria in Rapid Creek.  Murray, K.E., Kunza, L.A., DeVeaux, L.C.  Rapid City, SD.  March 15, 2017.  

 

Dakota Rural Action: 29th Annual Meeting.  What’s in Our River Water?  DeVeaux, L.C., Kunza, L.A., Murray, K.E.  Rapid City, SD.  October 8, 2016. 

 

South Dakota Public Health Association Conference, Keynote Address.  Establishing Gene Fingerprints of Pathogenic Bacteria in South Dakota.  DeVeaux, L.C., Kunza, L.A., Murray, K.E., Sioux Falls, SD.  June 29-30, 2016.

 

United States Geological Survey (USGS).  Pathogenicity Potential of Bacteria in Selected Locations along Rapid Creek and the Big Sioux River.  Murray, K.E., DeVeaux, L.C., Kunza, L.A.  Rapid City, SD.  March 4, 2016.

 

Wind Cave National Park, National Parks Service.  Coliform Bacterial Survey of Wind Cave National Park Perennial Streams with an External Review by South Dakota School of Mines and Technology.  Kunza, L.A., Murray, K.E., DeVeaux, L.C.  June 2, 2015.

Presentations              

 

Society for Freshwater Science.  Pathogenic Bacteria and Antibiotic Resistance in South Dakota Surface Waters.  Kunza, L.A., Murray, K.E., Preston, A., DeVeaux, L.C. Raleigh, NC.  June 8, 2017

 

8th Annual SDSMT Research Symposium.  Genetic Analyses of Potential Pathogenic Bacteria and Bacterial Communities Isolated from the Big Sioux River.  Murray, K.E., Kunza, L.A., DeVeaux, L.C.  Rapid City, SD.  April 4, 2017. 

 

West Dakota Water Development District.  DNA Characterization of Rapid Creek Bacterial Population.  Murray, K.E., Kunza, L.A., DeVeaux, L.C.  Rapid City, SD.  July 12, August 16, October 11, 2016.

 

Eastern South Dakota Water Conference.  Establishing Pathogenicity Profiles of Bacteria along Selected Reaches of Rapid Creek, Skunk Creek, and the Big Sioux River.  Murray, K.E., Kunza, L.A., DeVeaux, L.C.  Brookings, SD.  October 27, 2016. 

 

7th Annual SDSMT Research Symposium.   Establishing Gene Fingerprints of Pathogenic Bacteria along Selected Reaches of Rapid Creek.  Murray, K.E., Kunza, L.A., DeVeaux, L.C. Rapid City, SD.  March 31, 2016. 

 

East Dakota Water Development District.  Pathogenicity Potential of Bacteria in Selected Locations of the Big Sioux River.  Murray, K.E., DeVeaux, L.C., Kunza, L.A.  Sioux Falls, SD.  February 18, 2016.

  

The 319 Nonpoint Source Task Force Meeting.  Examining Seasonal Fluctuations in Pathogenicity Potential of the Big Sioux River and Skunk Creek.  Kunza, L.A, DeVeaux, L.C., Murray, K.E.  Pierre, SD.  December 8, 2015.

 

East Dakota Water Development District.  Pathogenicity Potential of the Bacteria in Selected Locations along the Big Sioux River.  Murray, K.E., DeVeaux, L.C., Kunza, L.A., Brookings SD.  June 17, 2015. 

 

Poster Presentations          

8th Annual SDSMT Research Symposium.  The Genetic Fingerprint of Bacteria found in Surface Water of the Big Sioux River.  Erickson, M.N., Murray, K.E., Kunza, L.A., DeVeaux, L.C..  Rapid City, SD.  April 4, 2017. 

 

2016 Western South Dakota Hydrology Conference: Second Place Poster Presentation.  Establishing Gene Fingerprints of Pathogenic Bacteria along Selected Reaches of Rapid Creek, Skunk Creek, and the Big Sioux River.  Murray, K.E., Kunza, L.A., DeVeaux, L.C.  Rapid City, SD.  April 15, 2016.

 

7th Annual SDSMT Research Symposium.  Pathogenicity Potential of Fundamental Regions of the Big Sioux River.  Sayler, S., Murray, K.E., DeVeaux, L.C., Kunza, L.A.  Rapid City, SD.  March 31, 2016. 

 

2015 Western South Dakota Hydrology Conference: First Place Poster Presentation.  Establishing Gene Fingerprints of Pathogenic Bacteria along Selected Reaches of Rapid Creek.  Murray, K.E., Kunza, L.A., DeVeaux, L.C.  Rapid City, SD.  April 15, 2015.

 

6th Annual SDSMT Research Symposium.  Gene Fingerprinting: A Method to Assess the Pathogenicity Potential of Microbes and Source of Contamination in Selected Reaches of Rapid Creek.  Murray, K.E., Kunza, L.A., DeVeaux, L.C.  Rapid City, SD.  March 31, 2015. 

Publications/Reports         

Murray, K.E.  Path-STREAM: Development and Implementation of a Novel Method for Determining Potential Risk from Pathogenic Bacteria in Surface Water Environments.  Ph.D. Dissertation, South Dakota School of Mines and Technology.        

 

Murray, K.E., DeVeaux, L.C.  Surface waters as environmental reservoirs of bacterial gene determinants carried by human pathogens: A review.  Science of the Total Environment.  In preparation. 

 

Murray, K.E., Erickson, M., Kunza, L.A., DeVeaux, L.C.  Pathogenicity Potential of the Bacteria in Selected Locations of the Big Sioux River and Skunk Creek.  East Dakota Water Development District Board and the City of Sioux Falls.  November 2017. 

 

Murray, K.E., Kunza, L.A., DeVeaux, L.C.  Establishing Gene Fingerprints of Pathogenic Bacteria along Selected Reaches of Rapid Creek.  Water Resources Institute.  March 2016.

Murray, K.E., DeVeaux, L.C., Kunza, L.A.  Pathogenicity Potential of the Bacterial in Selected Locations of the Big Sioux River.  East Dakota Water Development District Board.  March 2016.

 

Murray, K.E., DeVeaux, L.C.  Genetics Laboratory Manual for Instructors.  BIOL 371L.  Department of Chemistry and Applied Biological Sciences.  South Dakota School of Mines and Technology.  2015. 

          

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